![]() The Waukesha School District's ban on classroom signage considered too political forbids a colorful display of the district's own nondiscrimination policy, teachers say, because the reprint suggests a rainbow flag. A thin blue line flag indicates support for police but is also associated with opposition to racial justice.Įven a reprint of the district's own nondiscrimination policy – promising equality no matter students' race, religion, sexual orientation or learning ability – can't be posted because each word is a color of the rainbow, teachers said. ![]() Then the administration told teachers to remove LGBTQ+, Black Lives Matter, Blue Lives Matter, "safe space" and "thin blue line" American flag signage from classrooms. Board members said they received crude emails and even death threats from liberals. ![]() The board reversed that decision after a national uproar and local protests. Snapchat efforts to root out drug free#In August, the board voted to end a federally funded universal free meals program extended during the pandemic because some students weren't that poor and might become dependent on the assistance. "The intent is to come back to it," he said. One ran on a platform of no masks and ending COVID-19 quarantines.Īfter questions about the district's equity team that met monthly, the administration paused the work until it could provide more information to the board, Joe Koch, Waukesha's deputy superintendent, told USA TODAY. ![]() Two new conservative members won seats on the nine-person board with Republican Party support. In Waukesha, a conservative county directly west of Milwaukee where 60% of voters voted for then-President Donald Trump in 2020, the school board shifted further right in April. "As we hyperpoliticize the classroom and curriculum, there's a danger we'll eliminate exactly what students need (in order) to focus and learn." No 'safe spaces' in Waukesha Jackson Jr., dean of the communication school at the University of Pennsylvania. "This is a story about what’s happening in schools, but it's really about: Whose America is this?" said John L. We all have a unique perspective: Sign up for This Is America, a weekly take on the news from reporters from a range of backgrounds and experiences Restricting curricula and ignoring support for kids who are not white and straight sets back progress for all, they say. Progressives fear rolling back equity efforts is a harbinger for further controls on teachers and a less tolerant classroom environment. Conservatives feel that their ideology isn't well-represented in school rules or curricula and that a rebalancing is long overdue. Public schools are at an inflection point. "The Black Lives Matter organization is tied to a larger political movement, and with CRT, its logical end is more Marxist – more linked to central power, more government control and getting rid of the Constitution," said Sean Themea, chief of staff for Young Americans for Liberty, a libertarian student activist group based in Austin, Texas. Many conservatives believe addressing equity in that fashion amounts to liberal indoctrination – politics that should be removed from public schools. Waukesha School District students and teachers protest the district's ban on inclusive signage in classrooms, such as Pride flags and Black Lives Matter posters, before a school board meeting on Sept. In Wisconsin, Louisiana and Virginia, conservatives have gathered enough signatures to force recall elections of board members. Thousands more board seats will be up for grabs in November, giving people opposed to mask mandates, critical race theory and library books seen as too sexually explicit a chance to reshape their local schools. Real changes to kids' experiences at school have followed. School safety: Merrick Garland asks FBI to address threats of violence against board membersĪnd they ran for school board, many of them backed by national conservative organizations – and often, they won. Parents flooded school board meetings, sometimes becoming disruptive or even threatening. It started with parents observing their own kids' learning amid the coronavirus pandemic and grew as they became angry over rules about masks and vaccines. Others have jettisoned Black authors from reading lists and removed references to racial oppression and inequality in high school English literature curriculum. ![]() Some are overseeing the dissolution of diversity and equity committees and downplaying students' preferred personal pronouns. The district removed anti-racist books from reading lists before restoring them after student protests. 20 in Springettsbury Township, Pennsylvania. Central York High School teacher Patricia Jackson joins students Olivia Pituch, Renee Ellis, Edha Gupta and Christina Ellis for a demonstration Sept. ![]()
0 Comments
![]() Bitwarden vs lastpass password#Conclusionīitwarden by it self looks, from my perspective, a simple to use and more intuitive product, and it has everything you would expect from a good password manager. Or at least resetting it, to don't store any sensitive data in an inactive account. If you think you won't need your Lastpass account again I recommend you to delete it. So what reasons do I have to leave my Lastpass account with all of my passwords out there? Deleting Last Pass account?Īfter importing my passwords and testing out Bitwarden, and it's features definitely I won't need Last pass again. ![]() If you want to unlock Bitwarden with your bio metrics, go to Settings>Security>Unlock with biometrics. To enable the Autofill as the Last pass app does, go to Settings>Auto-fill> and check the Auto-fill service label. If you have any errors importing the best option is to read the bitwarden Documentation Installing the browser extension Now your Bitwarden's vault should have all of your passwords imported from Last pass. This time I'll do it in the online vault, but you can do it with the app, or extension too. So right after you import your passwords to Bitwarden (Or any password Manager), you must delete the password file. That means that your passwords won't be encrypted. WARNING: All of the data that is stored in that. ExportingĪfter that go to Advanced options>Manage your vault> ExportĬlick on that button and re enter your Lastpass master password.Īfter that, all of your passwords will be stored in. Exporting and Importing all of your passwordsĪt this point, you must login into your Last pass account and enter into your vault. Then you'll be redirected to your brand new Bitwarden vault.ĭon't forget to verify your email. ![]() Now that you've signed up, you have to login with the credentials. Since it's a master password you won't have any option to recover it.Įxample of a good MP: MykeyBOARD-is#brokenSINCE2020Īs you may notice it contains a lot of characters and numbers, but it's fully memorable, and there is a high probability of not forget it. Next you will be redirected to a signup page:Īdvice: Create a fully memorable, long and secure master password. Bitwarden vs lastpass free#So you'll need to scroll down up to the bottom and take a look at Bitwarden's pricing section.Īs you may notice all the options are guided to create an organization account, therefore if you want to create a personal account you must click in the personal plans tab.Īfter that, the personal plans menu will be shown, and now you can create a free account by clicking the create a free account button.ĭon't worry the paid plans are totally optional, and probably you'll get more of what you need with a free account. But currently the home page of Bitwarden don't show the create personal account button that clear. Migration processįirst you'll need to create a personal account in Bitwarden. So if you want to migrate your passwords, follow the following steps. 2FA, Auto fill forms, web extensions, mobile appįinally I get with a PM (Password manager), that filled out these requirements, Bitwarden Advantages of Bitwarden. Bitwarden vs lastpass full#It must be a full featured password manager:.I researched a lot about the bests password managers avaiable and I had two requirements: So this weekend I decided to migrate from Lastpass. Now you can only access your data either by Desktop or by the mobile app. Recently Lastpass has changed the way you can use it's free version. ![]() The problem comes when you analyze the free version of it. If you have a paid subscription I think you won't have any problems with it. So without further delay, let's get into the article. If you have some opinion about this post please let me know in the comment section. All is based in my opinion after reviewing these password managers and shouldn't be taken as a suggestion to use one product or another.Īs a developer I'm worried about the tools I use everyday and I took this space as a way of sharing my thoughts on it. I just wanted to share my experience, using different kind of tools. I'm not being benefited by any means by writing this article. ![]() ![]() ![]()
Sudo /home/user/Desktop/balenaEtcher-1.7.9-圆4. Near the top I see FUSE is for allowing non-root users to mount filesystems, so I tried running the previous command with sudo. #INSTALL LINUX BALENAETCHER INSTALL#It says Do not install FUSE on Ubuntu as of 22.04 or it may break your system. Plug in the other Tails USB stick that you want to install from. Shut down the computer while leaving the USB stick plugged in. You might still be able to extract the contents of this >AppImage if you run it with the -appimage-extract option. Make sure that you have installed Tails using either: balenaEtcher from macOS balenaEtcher from Windows GNOME Disks from Linux the Linux command line Shut down the computer. home/user/Desktop/balenaEtcher-1.7.9-圆4.AppImageĭlopen(): error loading libfuse.so.2 AppImages require FUSE to run. Tried to run it from the command line like this. AppImage but when I click on it there's no response. Press Enter to select a hard disk partition. ![]() Use the keys on the keyboard to navigate the list of partitions. Select the partition you want to install Arch Linux on. Installed Balena Etcher, unzipped, it's on my desktop as a. This displays a list of all disks and partitions along with the partitions for your other operating system. Got Mint iso installed, checksummed, ready to flash. We learned two methods to install Etcher using AppImage and CLI method.Hopping to Mint. #INSTALL LINUX BALENAETCHER HOW TO#In this tutorial, we learned how to install Etcher on Ubuntu. Once Etcher CLI installation is finished, open it from Ubuntu Software or run balena-etcher-electron in the terminal. #INSTALL LINUX BALENAETCHER UPDATE#Now add the repository key: $ sudo apt-key adv -keyserver -recv-keys 379CE192D401AB61įinally, update the repo and install Etcher: $ sudo apt update & sudo apt install balena-etcher-electron This is the preferred way if you wish to get Etcher updates automatically by settings up the repo via Software Updater or CLI.įirst, add the Etcher Ubuntu repository, type: echo "deb stable etcher" | sudo tee /etc/apt//balena-etcher.list Method 2: Install Etcher using Offcial Repo img, etc), choose the target device (usually a USB device), and click Flash to create a bootable USB. To use Etcher, first select the source image file (file format such as. Now you can Double click on the AppImage file to start the Etcher application. In the Permissions tab, check the 'Allow executing file as program' checkbox which gives execute permission for that file. Right-click on the AppImage file and select Properties. Select Target balenaEtcher guesses the intended target flash drive and presents its choice as the second step. There is also a Flash from URL option, though it’s not recommend to use that. #INSTALL LINUX BALENAETCHER ZIP FILE#zip file to a new directory named balena-etcher-electron-linux-圆4. Click the Flash from file button and select the ISO file that you downloaded earlier. Click on 'Download for Linux 圆4' button and choose Save File. You can simply download the file, give execute permission and run the application.ĪppImage doesn't need to install, compile or need root permission - apps are in read-only mode. Method 1: Install Etcher on Ubuntu using AppImageĮtcher Linux is available in AppImage package.
![]() ![]() But the movie turned out to be a straightforward, unremarkable caper that didn’t serve the actors well in the slightest, with de Armas’s Dani Miranda amounting to little more than a generic, no-nonsense agent with far less depth than de Armas is capable of illustrating. More than anything, the role proved that de Armas is a box office powerhouse, even if she’s barely in the movie! The Gray Man (2022)Īnthony and Joe Russo’s pulsing action thriller, The Gray Man, seemed poised to be a runaway hit for Netflix considering the brother directors’ proven success with their MCU offerings and the stacked main cast including Ryan Gosling, Chris Evans, and our girl de Armas. ![]() The only shame is that she’s in the film for only about 15 minutes despite being advertised heavily in the lead-up to the release. As superspy Paloma, she shatters Bond Girl tropes by proving to be 007’s equal at both kicking goon ass with style and looking insanely cool sipping on classy drinks at swanky bars. It’d be easy to write off de Armas as just another Bond girl in Cary Joji Fukunaga’s No Time to Die, but an actress of her caliber and range deserves more, and that’s what she got… sort of. This is all a narrative diversion, of course, and de Armas’s natural affability and magnetism help immensely to sell the ultimate twist while asking an important question: Can murder make you a better person? No Time to Die (2021) As the soft-spoken Marta, you’d never imagine she’d be involved in the Thrombey family’s bloody affairs to any extent, particularly because she’s surrounded by so many shifty characters that she gets lost in all the bickering and barbs. You wouldn’t know it at the movie’s outset, but de Armas emerges as the key player in Rian Johnson’s intricate whodunnit, Knives Out. The warmth and optimism she brings to the screen is essential to the film’s success, making the performance a must-watch for those new to de Armas’ work. “She shows more compassion and love and sacrifice than anybody else in the movie,” she told the El Paso Times back in 2017. ![]() The onscreen romance was as philosophically fascinating as it was visually stunning, and de Armas was nothing short of enchanting any time she graced the screen. But evidently, not even a language barrier could stop the runaway Ana de Armas hype train.ĭe Armas put on one of the most unforgettable performances in any sci-fi movie in recent memory as Joi, the holographic soulmate (or manipulative computer program, depending on who you ask) to Ryan Gosling’s K in Denis Villeneuve’s Blade Runner 2049. When de Armas was cast in Todd Phillips’ 2016 dramedy War Dogs alongside Jonah Hill and Miles Teller, she barely knew any English, telling The Hollywood Reporter, “I wasn’t really sure what I was saying” throughout her performance, and that she “learned lines phonetically.” It’s no small feat to make a splash in Hollywood for any actor or actress, let alone one who doesn’t even know enough to hold a conversation with her co-stars when the cameras aren’t rolling. ![]() Is there anything she can’t do? This wild-eyed performance would suggest not. But de Armas is absolutely riveting and shows her range, exuding innocence one moment and sheer insanity the next. It relies more on exploitation than provocation. The film is a bit contrived and spotty in how it’s laid out, making the social commentary less potent than it could have been. Valentine's Day Viewing Guide for Loners: 9 Shows and Movies for Single Folksīiggest Piracy Case in U.S.De Armas’s English-language debut was in Eli Roth’s psychosexual mind-fuck of a movie, Knock Knock, in which de Armas and Lorenza Izzo play underage girls who tease and then torture a hapless family man (Keanu Reeves) in his own home. 'Knock Knock' Star Ana de Armas to Play Miles Teller's Girlfriend in 'Arms and the Dudes' She next stars opposite Edgar Ramirez in “Hands of Stone” and recently reteamed with Reeves on “Daughter of God.” She’s represented by CAA and Impression Entertainment. executives Jesse Ehrman and Jon Gonda are overseeing the project for the studio.Īlso Read: ‘Jupiter Ascending': 9 Terrible Reviews That Might Knock the Sci-Fi Movie Out of Audiences Orbitĭe Armas played a convincing psychopath who tortures Keanu Reeves alongside Lorenza Izzo in Eli Roth’s “Knock Knock,” which Lionsgate acquired at Sundance. Bryan Zuriff is executive producing, while Warner Bros. Actors Ana de Armas, Keanu Reeves, Lorenza Izzo, Director Eli Roth and producer Aaron Burns attend the premiere of Lionsgates 'Knock Knock' at TCL Chinese 6 Theatres on Octoin. “Arms and the Dudes” is the first project produced under 22nd & Green, the new company run by Phillips and Bradley Cooper, who is producing with Mark Gordon. Jason Smilovic adapted the Rolling Stone article by Guy Lawson. ![]() ![]() ![]() It is strongly encouraged that all visitors evaluate their own health and any risks when planning a visit to El Pueblo Historical Monument, the Plaza Kiosko, and Olvera Street.Fever, New loss of taste or smell, Congestion or runny nose, Diarrhea, Chills, Nausea or vomiting, Fatigue, Sore throat, Shortness of breath/difficulty breathing, Headache, Cough, Muscle or body aches.Spanish or get yourself a translator and tell them you want read more. Visitors who have been exposed to confirmed COVID-19 cases and/or have experienced any of the following symptoms in the last fourteen (14) days are asked to not enter the Kiosko Plaza. Delivery & Pickup Options - 37 reviews of El Kiosko Located in the Value Village.All visitors must practice safe social (physical) distancing, which is defined as maintaining a minimum of six (6) feet distance between you and all others who are not current members of your household whenever possible. ![]() This face covering must be worn at all times while on property. #Kiosko trainslation freeIncludes free vocabulary trainer, verb tables and pronunciation. ![]() All visitors who enter the Plaza Kiosko must wear a face covering, unless under the age of 2. Look up the Spanish to English translation of kiosko in the PONS online dictionary. ![]() ![]() Shipments can only be done early in the morning when this case applies. Shipping through major International airports that support live animal shipments is accepted for transport. These papers will be provided to the airline for the shipping of your puppy. All of these are the papers necessary for your puppy to be shipped and travel across state lines. We are Select Breeders, you will also receive coupons/discounts on Pet Insurance, a shipping kennel, and USDA Veterinarian Health Certificate for shipping, acclamation certificate (note:) health certificate is from a certified veterinarian. In the crate, there will be a towel or baby blanket, a puppy toy, water, and food for your puppy. When shipped, your puppy will be sent in an airline-approved shipping crate. We certainly wouldn’t consider shipping if it wasn’t safe, It’s “almost” too easy, for everyone involved If there is a problem with shipping, I will do what I can to help, but it is your responsibility to deal with the airline to resolve any shipping or customs issues for your puppy. We ship our Yorkie puppies to all fifty states in the USA including Florida, Arizona, Arkansas, Colorado, Connecticut, California, Hawaii, Idaho, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, Nevada, New Hampshire, New Mexico, North Carolina, North Dakota, Oklahoma, Oregon, Pennsylvania, Rhode Island, South Dakota, Tennessee, Texas, Utah, Vermont, Washington, West Virginia, and Canada ![]() I have personally had a tour of the air terminal and plane facilities where my puppies are placed for their flight. These are the safest and only methods we use besides hand delivery. During June, July & August we sometimes opt to use night flights to provide comfortable travel temperatures. Priority Pet/VIP Service gives you “PRIORITY” This provides a climate-controlled pressurized environment as well as the quickest and most direct flight available. We always use Priority Pet Services for all of our dog/puppy air deliveries. ![]() We will ship puppies if and when the weather permits. Yorkie puppies for sale, Yorkies for sale, Yorkie for sale, Yorkies for sale near me, Yorkie poo for sale, Yorkies for sale near me, Yorkie poo for sale California Shipping is available (Shipping Prices and Policy) Yorkies are born black, gradually attaining their blue and tan coloration as they mature. The more attention this breed receives the better. They love to chase shadows, lay in sunbeams, and tug of war. Yorkies require minimal exercise and are suited for short walks and are ideal for apartment living or homes with small yards. They do not like to be ignored, so lots of praise will bring out the best in their training. Training must have consistency and firmness. However, they may become willful in which case training of any kind can become difficult. ![]() Unlike other breeds, which have fur, the Yorkshire has hair that is growing constantly. The Yorkie’s coat is not coarse but is fine and silky to the touch. However, if the Yorkie is not being used for show, then the coat may be clipped short. The hair on top of the head, if grown long, is usually secured with a band or bow. This breed’s coat requires daily grooming. The Yorkie can also be somewhat independent and assertive. ![]() They are tolerant of older children if treated with care. The Yorkie can be territorial and likes to have their space respected. It has been said that a Yorkie requires more human companionship and attention than any other breed. They focus entirely on their owner and are extremely affectionate. This dog is intelligent, lively, and fearless. They have a dose of attitude combined with a bright and playful nature and are considered a true and loyal companion. The Yorkie is a lovely lap dog that much prefers to be held on its owner’s lap all day. ![]() ![]() You have the right to cancel this contract within 14 days without giving any reason. You may cancel your order for any reason at any time prior to dispatch and make returns up to 30 days after receipt of your goods. Please note that these terms apply to products purchased through our website only. Plase note that all Store Folk items are fulfilled by a Third Part Seller on behalf of Jarrold. Please see the information box under the product name and price for delivery prices and times. Delivery Charges for items sold by Jarrold Store Folk partners If there is no postcode calculator, the item follows our standard delivery tariffs.įor more details on delivery please click here. Please use our postcode calculator on the product page to find out postage charges for the item. Scottish Highlands, Channel Islands and Northern Ireland S ee product page for delivery cost and estimate Select your day, on orders placed before 14:00 Parcelforce normally offer a one hour delivery window via SMS or Email.ĭelivery Charges for items sold by Jarrold Orders are sent with either Royal Mail and Parcelforce. We aim to dispatch all items which are in stock on the same working day when orders are received by 14:00. Please also expect to allow additional days for our standard delivery options around this period. We are sorry that our services will be impacted, and we will not be able to offer a Next Day or Nominated Day delivery service on certain days during this period. Do not modify darts or blaster.The Communication Workers Union (CWU) has called on its members at Royal Mail and Parcel Force, who collect, sort and deliver parcels and mail, to take strike action on the following dates: Other darts may not meet safety standards. Use of eyewear recommended for players and people within range. ![]() 6 NERF DARTS: Comes with 6 Official Nerf Elite foam darts that are great for outdoor and indoor battles and are tested and approved for performance and quality.SLAM-FIRE ACTION: Slam-fire all 6 darts fast! Hold down the trigger and keep moving the priming slide backward and forward to rapidly unleash 6 darts in a row.6-DART ROTATING DRUM: Fire 6 darts in a row from the blaster's rotating 6-dart drum designed so that the darts launch from the velociraptor's toothy jaws! Load, prime, and fire 1 dart at a time.NERF DINOSQUAD RAPTOR-SLASH BLASTER: Fire into ferocious fun with this dart blaster that features awesome dinosaur details, capturing the look of a Velociraptor dinosaur.Includes 6 Official Nerf Elite foam darts that are great for outdoor and indoor adventures. Pour on the firepower with slam-fire action - hold down the trigger and keep moving the slide backward and forward to release all 6 darts fast. Load the drum, move the priming slide backward and forward, and pull the trigger to unleash 1 dart. ![]() The blaster is designed so that the darts blast from the raptor's toothy mouth! Fire 1 dart a time, or slam-fire all 6 darts fast. The blaster features a 6-dart rotating drum so you can launch 6 darts in a row ![]() Team up with legendary dinosaurs as a member of the elite DinoSquad! Harness the extraordinary force of the greatest dinosaurs of all and blast into amazing adventures! Fire into ferocious fun with the Nerf DinoSquad Raptor-Slash blaster! It features an awesome dinosaur design, capturing the look of a Velociraptor dinosaur. ![]() ![]() ![]() Sheet Music Plus reserves the right to cancel or change this offer at any time.Offer ends at 11:59pm PDT on November 2, 2022. ![]()
![]() This is because Syncplay is intended to be used in conjunction with third-party communication solutions such as IRC and Mumble. Also, keep in mind that room names are case-sensitive. A complicated room name would keep random people from entering, i.e., serve as a password. Search chat rooms within the Internet Relay Chat and get informed about their users and topics Chat rooms on IRC are usually called channels. #Syncplay default room passwordIt is recommended to make the room name somewhat cryptic as a password doesn’t protect your server. Finally, Syncplay does not provide a voice or text-based chat platform to allow for discussion during playback. Lastly, the default room must also be the same for all the users. You can rate examples to help us improve the quality of examples. This means that when one person pauses/unpauses playback or seeks (jumps position) within their media player then this will be replicated across all media players connected to the same server and in the same 'room' (viewing session). These are the top rated real world Python examples of extracted from open source projects. Syncplay synchronises the position and play state of multiple media players so that the viewers can watch the same thing at the same time. Furthermore, users must manually choose what file to play as Syncplay does not synchronize which file is open. Python QLineEdit.hide - 3 examples found. Syncplay does not synchronize player configuration, audio/subtitle track choice, playback rate, volume, or filters. Syncplay does not use video streaming or file sharing, so each user must copy the media to be played. 5.0 3 years ago 7 Noob Player 2.4. Pausing, unpausing and jumping position within a media player is all synchronized, giving everyone connected to the same virtual room a shared. When a new person joins, they will also be synchronized. Syncplay synchronises video playback across multiple instances of VLC so that a group of people who all have the same videos can watch them together. This means that when one person pauses/unpauses playback or seeks (jumps position) within their media player, then this will be replicated across all media players connected to the same server and in the same 'room' (viewing session). ![]() Syncplay synchronizes the position and play-state of multiple media players so that the viewers can watch the same thing simultaneously. Place the a file in the main (all user) VLC /lua/intf/ sub-directory (or the single user directories). We've tried many different methods of watching movies together online, and they have all let us down (either requiring extra bloatware or only supporting youtube, or terrible performance, or not supporting Linux). ![]() ![]() #Syncplay default room installSyncplay can synchronize video playback on many players, including Mplayer, MPV, Media Player Classic, and VLC, so that you can watch movies with friends at the same time. Version 0.2.6 of the interface should be installed by default when you install Syncplay, but if it is not then you can install it manually. My friend and I have a weekly ritual of watching old kung-fu movies every Tuesday (aka Kung Fuesday). ![]() ![]() ![]() Everything we do is outlined in the blog and here, I can’t take the credit, actually I get zero credit, this is all my team, I just write about it. So there we have it, the highest-level overview of doing iPSC culture, but all the details are here. This is a stepwise freezing process, so start at -20 degrees, then -80 after a few hours, and then the next day to their final destination, the liquid nitrogen tank where it stays until you need it again, or until someone else needs it. Based on your numbers, pipette your cell suspension into multiple cryogenic vials, and then voila, ready for the freezer. Once you have your cell pellet, resuspend in freeze down media, and calculate the cell viability and total cell number. Now for freezing cells, its pretty much the same as passaging, with one small exception, this is the end of the cells current journey as they are heading to the liquid nitrogen tank in the big storage room down the hall. You better pray someone else does, or your best bet is to simply freeze the cells down. If you don’t have a coated dish, then gulp. This plate is coated like we describe in Tuesday’s blog. These cells are pelleted, resuspended in media, and then carefully, pipetted to break the aggregates down to add onto a new plate. To do so, we use gentle cell dissociation media to lift the cells from the plates. This means removing the cells from the dish they are on, to put a small amount of these cells onto a new dish to grow again. Once the cells have reached critical mass and in our SOP, we have recommendations on when this is, then you are ready to passage. Images of what this looks like are below, monitor daily for the bad cells, when you see them try to remove them, it helps in keeping the cells all nice and pluripotent. This is like when they lose their superpowers and are no longer a superhero but just a regular person, or in stem cell terms, they lose their pluripotency. Also look out for differentiation, or when the cells become bad. But the growth rate can vary from line to line, and depends on the media you use, so be mindful of this when doing your cultures. As your cells grow, they will form tightly packed colonies that grow and expand in size over about 6-7 days until they reach confluency. There are some new approaches in which you can skips days but the preference for our group is daily media changes. ![]() How all this works is in the SOP we published on Tuesday. ![]() Also, every day you need to change the media. Your cells are attached and growing, so every day you must now keep an eye on them to make sure they are happy. Ok, enough of the fun and games, lets get down to serious business. As you can see, blue has a special place in my heart looking after my stress ball brain, he sits in on all my meetings giving constructive advice as we all work to take a bite out of the modern scientific problems of our day (haha, sorry that was an awful pun, please forgive me). But thank you for asking, they are doing well and no they don’t have their own instagram account, here is my first and last picture of my collection as they like their privacy. Before I get started, I have been inundated with requests from people asking how the dinosaurs are doing, I don’t know if they are mocking me, or if they are serious. As promised, the continuation of Tuesday’s blog in which we learn to maintain iPSCs, to split iPSCs, and yes, as it says it in the title, to freeze down iPSCs. But when its hot, what better way to cool down then with an ice cold beverage (I mean freshly frozen tube of stem cells). Man, it is a scorcher today, with a humidex of really humid, now I know how cells feel like living in an incubator. In the immortal words of Ron Burgundy “ Its so hot… milk was a bad choice”. ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |